Terminator
Rbcs2B5-C1

Part:BBa_K2984018:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)


RbcS2 - Terminator B5-C1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was designed as a B5-C1 part according to the MoClo standard of Patron et al. (2015).


Source

Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. J Mol Biol, 191(3), 421-432. doi:10.1016/0022-2836(86)90137-3. Stevens, D. R., Rochaix, J. D., & Purton, S. (1996). The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas. Mol Gen Genet, 251(1), 23-30. doi:10.1007/bf02174340. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.


References